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The purpose of this position is to quantitate drug concentrations for anti-human immunodeficiency virus (HIV) and anti-tuberculosis (TB) medications, antibiotics, and controlled substances primarily in human hair, dried blood spot (DBS), and urine samples (but may include other biological specimens, such as whole blood, plasma, serum, nail, saliva, etc.), utilizing high-performance liquid chromatography (HPLC)-tandem mass spectrometry (LC/MS/MS) analytical methods for pharmacokinetic, compliance, and efficacy studies. Laboratory duties include, but not limited to, logging in samples via a laboratory data management system (LDMS); sample preparation; operating and calibrating analytical instruments; using and calibrating micropipettes; thorough documentation in lab books, log books, and paperwork; and the actual analysis (and subsequent interpretation) of data. The successful candidate will also assist in the validation of LC/MS/MS methods for antiretroviral and anti-TB drug analysis in hair, but may also include other biological specimens, in compliance with the Food and Drug Administration's (FDA's) Good Laboratory Practice (GLP) and National Institute of Health's (NIH's) Division of AIDS' (DAIDS) Clinical Pharmacology Quality Assurance (CPQA) regulatory guidelines. The UCSF Hair Analytical Laboratory (HAL) analyzes antiretroviral, anti-tuberculosis (anti-TB), antibiotic, and controlled substance concentrations in hair (but may include other biological specimens such as urine, dried blood spot (DBS), plasma, serum, whole blood, nail, saliva, etc.) in the context of HIV and TB prevention and treatment studies using assays that are developed and validated according to the Federal Food and Drug Administration's (FDA's) guidelines for bioanalytical methods and the National Institute of Health's (NIH's) Division of AIDS' (DAIDS) Clinical Pharmacology Quality Assurance Program (CPQA). This position is responsible for overseeing and maintaining high-quality regulatory functions in the laboratory. This HAL Staff Research Associate I position will assist senior analysts in determining drug concentrations in samples utilizing validated high-performance liquid chromatographic (HPLC)-tandem mass spectrometric (LC-MS/MS) bioanalytical methods. DUTIES & ESSENTIAL JOB FUNCTIONS Identify the functions or tasks that employees in the job perform. The essential functions should state the purpose of the work and the results to be accomplished, rather than how the function is performed. Of the tasks listed, what percentage of time is devoted to each? The more time employees spend on a function, the more likely it is that the function is essential. Generally, include those functions that account for 10% or more of the work, i.e., key items that contribute significantly to the achievement of the job. The functions should add up to 100%.
% of time |
Essential Function (Yes/No) |
Key Responsibilities (To be completed by Supervisor) |
30 |
Yes |
Preparation of hair samples (but may include other biological specimens, such as urine, dried blood spot (DBS), plasma, serum, whole blood, nail, serum, etc.) to determine drug concentrations, which involves measuring and cutting hair samples into very small pieces, weighing and processing the hair utilizing analytical methods including protein precipitation, liquid-liquid extraction, solid-phase extraction, among other methods to run and analyze on a high performance liquid chromatography (HPLC)-tandem mass spectrometer (LC-MS/MS). Other responsibilities during sample preparation include pipetting and transferring microliter volumes of solutions following standard operating procedures (SOPs); weighing milligram amounts of chemicals using a microbalance; preparing standard solutions using volumetric flasks, reagents, mobile phases, and solvent mixtures; comparing standard solutions utilizing HPLC-ultraviolet detector (HPLC-UV); documenting daily tasks into laboratory notebooks and log books in accordance with SOPs; and filing paperwork and lab books into the appropriate locked drawers. Other duties may include dilution of urine specimens in order to analyze tenofovir (an HIV antiretroviral medication) levels in urine using LC-MS/MS. |
20 |
Yes |
Operation of the HPLC-UV and LC-MS/MS equipment including preparing and loading batch files into the system; loading samples into the autosampler; choosing appropriate method, mobile phase(s), needle wash, and analytical column according to the analytical procedure (AP) of the compound being measured; calculating/interpreting the generated data; and summarizing the data into Excel tables as final data to send to sponsors and include in analytical reports. |
20 |
Yes |
Assist in method validation using LC-MS/MS, help prepare SOPs and analytical validation reports (AVRs) in compliance with the Food and Drug Administration's (FDA's) and the National Institute of Health's (NIH's) Division of AIDS' (DAIDS) Clinical Pharmacology Quality Assurance (CPQA) regulatory guidelines, and assist in responding to CPQA inquiries about the SOP and AVR reports. |
15 |
Yes |
Assist in other laboratory tasks as needed, but not limited to, washing and organizing glassware; re-stocking and re-organizing supplies; calibrating the microbalances, micropipettes, repeater pipette, and equipment; maintaining and troubleshooting equipment with the assistance of other staff members and management; collecting positive quality control samples; and checking, printing, and filing iMonnit freezer/refrigerator charts. |
10 |
Yes |
Utilize laboratory information management system (LDMS) for scanning and logging in samples received by the laboratory for specimen tracking and inventory storage, update LDMS inquiries from clinical laboratories, and contact the appropriate personnel for discrepancy queries. |
5 |
Yes |
Contributes towards and/or writes articles for publication in scientific journals or for presentations at conferences or symposiums. |
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100% |
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(To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.) |
Required Qualifications
- BA/BS degree in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience
- Basic related scientific techniques and procedures as learned from academic laboratory or field experimental experience
- Computer software skills (MS Word, Excel and PowerPoint)
Preferred Qualifications
- Strong basic laboratory techniques, such as micropipetting; measuring solutions using volumetric flasks and graduated cylinders; making reagents, mobile phases, and standard solutions; using centrifuges, vortexers, and other equipment in an analytical laboratory
- Thorough documentation of daily procedures into lab books and log books
- Basic HPLC and LC/MS/MS operation experience
Required Qualifications
- BA/BS degree in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience
- Basic related scientific techniques and procedures as learned from academic laboratory or field experimental experience
- Computer software skills (MS Word, Excel and PowerPoint)
Preferred Qualifications
- Strong basic laboratory techniques, such as micropipetting; measuring solutions using volumetric flasks and graduated cylinders; making reagents, mobile phases, and standard solutions; using centrifuges, vortexers, and other equipment in an analytical laboratory
- Thorough documentation of daily procedures into lab books and log books
- Basic HPLC and LC/MS/MS operation experience
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University of California - San Francisco
Unknown
Jun 10, 2026