Laboratory Technician III

M-F 8-5 w/ 1 hour for lunch

Hanover, NH 03755
702 Remsen Building

To help manage the lab and conduct a variety of experiments in support of research studies on brain development and behavior using the zebrafish model. The position will involve general lab duties, preparing constructs, and recovering and applying transgenic tools.

The ideal candidate will be familiar with genetics and neurobiology and have

experience with molecular and cell biology techniques, including cloning and PCR,

transcriptomics, image analysis, and/or microscopy. We seek a creative and motivated

scientist who works well independently and as part of a team. This position will entail

independent research, zebrafish husbandry, and technical training of lab members.

* Competence in laboratory methods, procedures, and techniques as well as equipment and instruments (e.g., gel electrophoresis, cloning, PCR, microscopes).
* Reliable and self-motivated.
* Ability to follow directions and work independently with good judgment.
* Strong organizational and time management skills.
* Willingness to work with/be exposed to hazardous chemicals.

* Knowledge of computers and software applications.
* At least one year of relevant laboratory or research experience.
* Some expertise in molecular biology is desirable.

Dartmouth College is an equal opportunity employer under federal law. We prohibit discrimination on the basis of race, color, religion, sex, age, national origin, sexual orientation, gender identity or expression, disability, veteran status, marital status, or any other legally protected status. Applications are welcome from all.

Employment in this position is contingent upon consent to and successful completion of a pre-employment background check, which may include a criminal background check, reference checks, verification of work history, conduct review, and verification of any required academic credentials, licenses, and/or certifications, with results acceptable to Dartmouth College. A criminal conviction will not automatically disqualify an applicant from employment. Background check information will be used in a confidential, non-discriminatory manner consistent with state and federal law.

This position is funded by a grant. Continuation is based upon available funds.

Dartmouth College has a Tobacco-Free Policy. Smoking and the use of tobacco-based products (including smokeless tobacco) are prohibited in all facilities, grounds, vehicles or other areas owned, operated or occupied by Dartmouth College with no exceptions. For details, please see our policy. https://policies.dartmouth.edu/policy/tobacco-free-policy

* Accomplishes day-to-day care and housekeeping of laboratory, including maintaining clean and organized working environment

* Orders laboratory supplies, prepares reagents and solutions as needed.

* Monitors laboratory equipment and instrument performance; obtains maintenance and repair as necessary.

* Maintains computerized records or detailed notes associated with laboratory activities and sends requested reagents to other researchers

* Instructs others in laboratory methods, procedures, and techniques and provides technical guidance and direction as required.

* Upon training, performs animal husbandry duties specific to the zebrafish system.

* Conducts specified laboratory experiments using a range of established molecular biology and genetic methods, procedures, and techniques (e.g., generation of constructs and transgenic lines, CRISPR/Cas9 genome editing, PCR, etc.).

* Monitors experiments while in progress; records observations

* Reconciles results that are disparate from anticipated findings; trouble-shoots and re-runs experiments as necessary.

* Collects and analyzes data resulting from experiments and discusses findings with Principal Investigator as well as potential problems associated with experiments, measurements, or analyses and suggests changes as appropriate.

* Adheres to laboratory safety requirements including general and chemical/hazardous waste safety disposal methods

* Handles and cares for zebrafish, including collecting tissue, breeding, collecting

embryos, washing tanks.

* Uses PCR and molecular cloning methods to identify mutations in zebrafish lines

and maintain zebrafish stocks.

* May assist Aquatics Facility staff with feeding and facility maintenance as needed.

* Maintains laboratory notebook to record tasks performed.

* Contributes to the preparation of scientific manuscripts where appropriate (providing data such as graphs, charts, tables, statistical summaries, etc.).

* Oversees entries into lab databases.

* Conducts literature searches to develop insights into the significance of experimental results and to acquire information on new or improved methods, procedures, and techniques.